G1733A Polymorphism of the Androgen Receptor Gene and Recurrent Spontaneous Abortion in Saudi’s Women

Department of Zoology, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia Department of Obstetrics and Gynecology, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia Molecular Biology Division, Zoology Department, Faculty of Science, Alexandria University, Alexandria, Egypt *Corresponding Author’s Email: [email protected] Tel: +966507201601 Various gene polymorphisms have been reported to influence the risk of recurrent spontaneous abortion (RSA). In females, androgen receptor (AR) is expressed in reproductive tissues; however, the causal association of androgens receptors (AR: G1733A) in RSA has not been investigated in Saudi’s women with RSA. The aim of the present study was to investigate the potential effect of the G1733A polymorphism of the AR gene in the occurrence of RSA in Saudi women. The study group comprised of 65 Saudi women with unexplained RSA. The reference population consisted of 65 Saudi women who had at least 3 children, and were without known pregnancy losses or any known medical illnesses. Peripheral venous puncture, DNA extraction, PCR, and sequencing were employed to genotype women for the presence of a polymorphism at position G1733A of androgen receptor gene. For the G1733A polymorphism, the frequency of the A allele in the Saudi healthy females is 16.2%. The frequencies of homozygous AA were 6.2% and 0% in the control and RSA group, respectively, but the difference was not significant (p>0.05). In addition the frequency of heterozygous GA was 20% for the control group and 26.2% for the patient group but the difference was not significant (P>0.05). Regarding the G1729A polymorphism, the frequency of the A allele in the Saudi females was 3.1%. The frequencies of GA+AA were 6.2% and 1.5% in the control and patient groups, respectively, and the difference was not significant (p>0.05). These findings confirmed that there were no causal association between the occurrence of the A allele of the G1733A and G1729A polymorphisms of the AR gene and increased risk of RSA in Saudi’s women.